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Maintain clones at low-copy number
an alternative to SuperScript® II Reverse Transcriptase
As expected
forming oligoribonucleotides having 3´-terminal pyrimidine-3´-phosphates
where there is a 2´
RNase I, 1,000 U size:EACH Maintain clones at low-copy numberRemove unwanted RNA by digesting with this heat labile, non sequence specific ribonuclease Complete: Unlike RNase A, RNase I does not have a base preference and digests between all dinucleotide pairs in single stranded RNA Heat Labile: RNase I is easily inactivated by heating 70C for 15 minutes RNase I degrades single stranded RNA to nucleoside 3 monophosphates via 2,3 cyclic monophosphate intermediates by cleaving between all dinucleotide pairs,2,3
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